Extracellular Vesicle Core Fee Structure
Effective March 1, 2024: The following prices are for Principal Investigators at CHLA and USC; rates are subject to change in the future.
Service | Instrument / Notes | Rates for CHLA |
---|---|---|
Nanosight Tracking Analysis (NTA) - Full Service | NanoSight NS300 | $125/hour |
Nanoparticle Tracking Analysis (NTA) - Trained User | NanoSight NS300 | $50/hour |
Extracellular Vesicle Isolation from cell culture supernatants, includes NTA analysis | Single isolation method: Ultrafiltration/Tangential Flow Filtration followed by Size Exclusion Chromatography; fee varies based on starting volume | Up to 150ml: $152/sample |
Extracellular Vesicle Isolation from Biofluids/Tissues (e.g., plasma/serum), includes NTA analysis. | Double isolation method: Density Gradient Ultracentrifugation followed by Size Exclusion Chromatography | $192.50/sample |
ExoView - Full Service | ExoView R100 | $164/sample |
ExoView - User-assisted (user provides chips) | ExoView R100 | $87/sample |
Large scale extracellular vesicle production in bioreactor | Hollow-Fiber Bioreactor system. Pricing for small, medium and large cartridges, maintenance is per week. | Setup: $773/$926/$$1159 Maintenance: $214/$273/$346 |
RNA Isolation | QC by Nanodrop; includes DNAse or RNAse treatment (when requested for EV samples) | $18/sample |
qPCR full service | BIoRad CFX96 Touch | $2.40/replicate |
Instrument Use Only | BIoRad CFX96 Touch | $20/hour |
Immunoblotting for EV characterization | According to ISEV guidelines - Opens in a new window | $294/gel |
EV-depleted FBS | 50ml aliquots | $89.90/aliquot |
Use of Tangential Flow Filtration System (user provides filters and other supplies) | KrosFlo TFF by SpectrumLabs | Complimentary |
Use of Ultracentrifuge - requires IBC and Biohazard use approval | Beckman Coulter Optima XE-90K | Complimentary |
Generation of empty lipid nanoparticles (LNP) | 18ml aliquot | $164/aliquot |
Labeling of up to 500ug of antibody | User provides functional grade antibody | $55/labeling |
Loading of LNP with nucleic acids and antibody | User provides nucleic acid and antibody | $33/reaction |
User Accessible Chromatography system (user provides all supplies) | ÄKTA start – Instrument use only | $10/hour (up to 4 hours) |
TaqMan advanced miRNA array | TaqMan qPCR-based screening of 754 human miRNAs (click for more info - Opens in a new window) with as low as 1ng of RNA | $727/sample |
Instrument training fee | Required for: | $125/hour |
Technical Assistance | Consultation/analysis and experiment assistance | $87.90/hour |
Core Reagent Inventory
Select Cores offer reagents as part of their purchasable services to the CHLA research community. A searchable Core Reagent Inventory was created in support of the CHLA research community BenchResearchHelp - Opens in a new window initiative that connects researchers with reagents when in need of a small amount of a reagent, such as an antibody, to determine the efficacy in a particular experiment.
CHLA based investigators are welcomed to browse the Cores Reagent Inventory - Opens in a new window to access the complete product list for pricing and availability. The database will be updated on an annual basis. Contact the Core for more information.