Extracellular Vesicle Core Publications and Data
How to cite the Core in publications
The EV work was performed in the Extracellular Vesicle Core at The Saban Research Institute, Children's Hospital Los Angeles.
Publications made possible by Core support
Pike S, Peng CC, Neviani P, Berry JL, Xu L. CD63/81 Small Extracellular Vesicles in the Aqueous Humor are Retinoblastoma Associated. Invest Ophthalmol Vis Sci. 2023 Jul 3;64(10):5. doi:10.1167/iovs.64.10.5. PMID: 37410475; PMCID: PMC10337798. https://pubmed.ncbi.nlm.nih.gov/37410475/
Blavier L, Nakata R, Neviani P, Sharma K, Shimada H, Benedicto A, Matei I, Lyden D, DeClerck YA. The capture of extracellular vesicles endogenously released by xenotransplanted tumours induces an inflammatory reaction in the premetastatic niche. J Extracell Vesicles. 2023 May;12(5):e12326. doi: 10.1002/jev2.12326. PMID: 37194998; PMCID: PMC10190125. https://pubmed.ncbi.nlm.nih.gov/37194998/
Peng CC, Im D, Sirivolu S, Reiser B, Nagiel A, Neviani P, Xu L, Berry JL. Single vesicle analysis of aqueous humor in pediatric ocular diseases reveals eye specific CD63-dominant subpopulations. J Extracell Biol. 2022 Apr;1(4):e36. doi: 10.1002/jex2.36. Epub 2022 Apr 20. PMID: 36339649
Xin Tang, Cheng Chang, Jiacong Guo, Vadim Lincoln, Mei Chen, David T. Woodley, and Wei Li. Department of Dermatology and the Norris Comprehensive Cancer Centre, University of Southern California Keck Medical Centre. Tumour-Secreted Hsp90a on the External Surface of Exosomes Mediates Tumour-Stromal Cell Communication via Autocrine and Paracrine Mechanisms. Sci Rep. 2019 Oct 22;9(1):15108
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805946/
Paolo Neviani, Petra M Wise, Mariam Murtadha, Cathy W Liu, Chun-hua Wu, Ambrose Y Jong, Robert CSeeger and Muller Fabbri Natural Killer-derived exosomal miR-186 inhibits neuroblastoma growth and immune escape mechanisms. Cancer Res December 12 2018 DOI: 10.1158/0008-5472.CAN-18-0779
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428417/
References and Useful Publications
- Considerations on how to pick the best isolation methods.
Method | Advantages | Disadvantages |
|---|---|---|
Differential ultracentrifugation | Standard method; most commonly used until recently. Isolation from large volumes. | Lengthy (4-5h). |
Density Gradient on Sucrose or Iodixanol cushion | Highest degree of purity based on density. | Very lengthy (16+ hours), very sensitive to centrifugation time. Complex. Not easily scalable. Sucrose toxicity. |
Size Exclusion Chromatography | Highest degree of purification based on size; vesicles not affected by shearing force, gentle. Large scale. Superior reproducibility. | Highest degree of purification based on size; vesicles not affected by shearing force, gentle. Large scale. Superior reproducibility. |
Immuno-affinity sorting | High purity based on surface markers | Not suitable for large samples; low yield. |
- Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. Thery et al, JEV. 2018 Nov 23;7(1):1535750. doi: 10.1080/20013078.2018.1535750 (https://www.tandfonline.com/doi/full/10.1080/20013078.2018.1535750)
