Stem Cell Core Equipment and Services

The Stem Cell Core offers cell distribution, biobanking and stem cell testing services, assisting both Children’s Hospital Los Angeles investigators and external investigators with their research.

The Stem Cell Core offers:

• Human pluripotent stem cell line (hPSC) distribution: WA01,WA09
• Custom human iPSC reprogramming from blood or fibroblasts
• Maintenance of investigator iPSC lines
• hPSC culture training (feeder or feeder free)
• General tissue culture training
• Mycoplasma testing
• Test for Identification of Undifferentiated cells
• Genetic testing by PCR via Stem Genomics
• STR cell line authentication via University of Arizona
• Rental of fully equipped tissue culture facilities

Stem Cell Core can help investigators using the Core with supplies management, including ordering, stocking and keeping inventory, and they can provide tissue culture supplies, like pluripotent stem cell related reagents, at a discount through bulk purchasing.

The following equipment is available within the Stem Cell Core:

• QuantStudio™ 7 Flex Real-Time PCR System with fast 96-well, 384-well, TLDA card blocks
• Nucleofector II with or without Lonza 96-well shuttle
• Dissection microscopes
• TS100 Fluorescent microscope
• Countess automated cell counter
• Liquid nitrogen and -80 freezer storage space

QuantStudio™ 7 Flex Real-Time PCR System with fast 96-well, 384-well, TLDA card blocks

Amaxa Nucleofector II with or without Lonza 96-well shuttle

Nikon dissection microscopes

TS100 Fluorescent microscope

Countess automated cell counter

Liquid nitrogen and -80 freezer storage space